Objective
Cell self-polarization and directional migration is a well-observed phenomenon, but despite its applications in tissue engineering, immunology, and cancer cell biology the phenomena is poorly characterized–especially in 3D microenvironments. We aim to use fluorescent light sheet microscopy in conjunction with FRET-based talin tension-sensing fibroblasts in tunable fibrin hydrogels to characterize cells’ dynamic, 3D spreading and force generation behaviors. Fibroblasts with FRET-based talin force sensors enable the characterization of cells’ dynamic force generation. This data will be correlated with spreading behaviors to examine corresponding force generation between actin and the extracellular matrix. In this way, we aim to establish and validate a platform that spatiotemporally characterizes cells’ dynamic mechanotransduction pathways.
Expected Outcome
Project Details
Budget
$225 000
Timeline
1 August 2023 - 31 July 2026
Slide Deck
Project Updates/ Results
As a result of this project, we aim to better understand the dynamics behind cell force generation and self-polarization. This improved understanding will enable other researchers to better understand the underlying mechanisms behind cell-ECM interactions in morphogenesis, directed cell motility, and cancer cell metastasis. Finally, we anticipate that his project will provide a platform to investigate how cells internalize mechanical cues from their ECM and how these pathways can be promoted or inhibited.
List of Project Publications
NA
The Experts & Partners involved with this Project
Robert Tranquillo
Distinguished McKnight University Professor, Department of Biomedical Engineering
Meghan Driscoll
Assistant Professor
Adam Ley
Biomedical Engineering